CONTROL OF GENE ACTIVITY IN HIGHER EUKARYOTIC CELLS BY PROKARYOTIC REGULATORY ELEMENTS

Regulated gene expression systems for the study of gene function in prokaryotes and yeast have been available for several years. By contrast, comparable systems in higher organisms have proven to be problematic due to their high complexity. Recently, regulatory proteins from distant species have been used to establish highly specific control systems in eukaryotic cells. This is possible because their action can be modulated by effecters that are inert to the physiology of the cell or organism and therefore do not elicit pleiotropic effects. Such monospecific regulatory circuits open up new possibilities for the study of gene function in vivo.