A SIMPLE AND ROBUST HPLC METHOD FOR THE DETERMINATION OF PAROXETINE IN HUMAN PLASMA

被引：28

作者：

KNOELLER, J

VOGTSCHENKEL, R

BRETT, MA

机构：

[1] Focus Clinical Drug Development GmbH, 41460 Neuss

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1995年 / 13卷 / 4-5期

关键词：

PAROXETINE; HPLC; PLASMA; ASSAY VALIDATION;

D O I：

10.1016/0731-7085(95)01315-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The objective of this investigation was to establish and validate an HPLC method with UV detection for the determination of paroxetine in human plasma. The method was validated in the concentration range from 6 to 100 ng ml(-1). The lower limit of reliable quantification (LLQ) was 6 ng ml(-1). The extraction efficiency varied from 67.1 to 85.5% over this range. Accuracies calculated at three concentrations in each of three separate runs were between 99.4 and 109.6%, and precision data were between 1.86 and 9.1%. The means of the between-day precision at all concentrations were between 2.77 and 7.32%. The corresponding means of the accuracy data were in the region of 102.4 to 106.3%.

引用

页码：635 / 638

页数：4

共 3 条

[1] DETERMINATION OF PAROXETINE IN HUMAN-PLASMA, USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION [J].