BACILLUS-AMYLOLIQUEFACIENS POSSESSES A 2ND TYPE-I SIGNAL PEPTIDASE WITH EXTENSIVE SEQUENCE SIMILARITY TO OTHER BACILLUS SPASES

被引：18

作者：

HOANG, V ^{[1
]}

HOFEMEISTER, J ^{[1
]}

机构：

[1] INST PLANT GENET & CROP PLANT RES, DEPT MOLEC GENET, D-06466 GATERSLEBEN, GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1269卷 / 01期

关键词：

SIGNAL (LEADER) PEPTIDASE TYPE I; SIP GENE; PCR CLONING; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; FRUA GENE; (BACILLUS);

D O I：

10.1016/0167-4889(95)00101-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A second sipS2(BA) gene was PCR cloned from Bacillus amyloliquefaciens. The deduced aa sequence is similar to those of the SPases of B. subtilis, B. amyloliquefaciens, and B. licheniformis and the domain structure of the gene has been preserved, A low Level of monocistronic gene transcription could be shown using Northern analysis. The sipS2(BA) gene was mapped to a region downstream of an E. coli fruA gene homologue and shown to express a 21 kDa protein in Escherichia coli.

引用

页码：64 / 68

页数：5

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