SPECIFIC LABELING OF A PHOSPHATE-TRANSPORTING PROTEIN FROM RAT-LIVER MITOCHONDRIA BY [MERSALYL-HG-203

被引：20

作者：

HOFMANN, HD

KADENBACH, B

机构：

[1] Biochemie, Fachbereich Chemie der Philipps, Universität Marburg, Marburg, D-3550, Lahn

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 102卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb04278.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly specific labeling of a phosphate‐transporting protein from rat‐liver mitochondria was obtained with [203Hg]mersalyl under the following conditions: (a) labeling of mitoplasts, isolated by the French‐Press procedure; (b) washing of the isolated inner membranes with NaCl/octylglucoside. The concentration‐dependent labeling of the protein with mersalyl showed a biphasic saturation which correlated well with the protection and inhibition of phosphate transport. The molecular weight (Mr 31000) and the amount of labeled carrier protein (24 nmol/g mitochondrial protein) at 100 protection correlated well with published data from the literature. Gel filtration of the labeled protein in the presence of non‐ionic detergents showed a molecular weight of at least twice of that obtained by gel electrophoresis in the presence of sodium dodecylsulfate. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：605 / 613

页数：9

共 43 条

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