THE INHIBITION OF THROMBOPLASTIN BY APOLIPOPROTEIN-B AND THE EFFECT OF VARIOUS ASSOCIATED LIPIDS

被引：10

作者：

ETTELAIE, C ^{[1
]}

HOWELL, RM ^{[1
]}

机构：

[1] KINGS COLL LONDON, DIV BIOMOLEC SCI, BIOCHEM SECT, CAMPDEN HILL RD, LONDON W8 7AH, ENGLAND

来源：

THROMBOSIS RESEARCH | 1992年 / 68卷 / 02期

关键词：

THROMBOPLASTIN; INHIBITION; APOLIPOPROTEIN-B;

D O I：

10.1016/0049-3848(92)90033-7

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

Human apolipoprotein B was purified by barium sulphate adsorption, subsequent delipidation and gel filtration. The protein was then reconstituted in soya bean lecithin and its inhibitory effect towards thromboplastin was assayed by incubation with rabbit brain thromboplastin. The use of an antibody against human apolipoprotein B diminished this inhibition. The level of inhibition of thromboplastin by the reconstituted apolipoprotein was found to be dependent on the concentration of the phospholipids with which it was reconstituted, reaching a maximum inhibition at a lipid : protein ratio of 1:1 (w/w). However, higher phospholipid concentrations or inclusion of cholesterol esters or triglycerides diminished and at certain concentrations reversed the inhibitory effect of the apolipoprotein. These results point towards apolipoprotein B as an inhibitor whose activity towards thromboplastin could be dependent on the complexes it forms with the surrounding lipids.

引用

页码：175 / 184

页数：10

共 36 条

[1]

DEGRADATION OF CATIONIZED LOW-DENSITY LIPOPROTEIN AND REGULATION OF CHOLESTEROL-METABOLISM IN HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA FIBROBLASTS [J].

BASU, SK ;

GOLDSTEIN, JL ;

ANDERSON, RGW ;

BROWN, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (09) :3178-3182

[2]

SYNCHROTRON X-RAY AND NEUTRON SOLUTION SCATTERING STUDIES OF STRUCTURAL-CHANGES IN LOW-DENSITY LIPOPROTEINS [J].

BELLAMY, MF ;

NEALIS, AS ;

AITKEN, J ;

BRUCKDORFER, KR ;

PERKINS, SJ .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1989, 17 (04) :680-681

[3]

BROZE GJ, 1987, BLOOD, V69, P150

[4]

MODIFICATION OF EPSILON-AMINO GROUP OF LYSINES, CHOLESTEROL OXIDATION AND OXIDIZED LIPID APOPROTEIN CROSS-LINK FORMATION BY PORPHYRIN-PHOTOSENSITIZED OXIDATION OF HUMAN LOW-DENSITY LIPOPROTEINS [J].

CANDIDE, C ;

REYFTMANN, JP ;

SANTUS, R ;

MAZIERE, JC ;

MORLIERE, P ;

GOLDSTEIN, S .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1988, 48 (02) :137-146

[5]

INVITRO INTERACTION OF THE PHOTOACTIVE ANTICANCER PORPHYRIN DERIVATIVE PHOTOFRIN-II WITH LOW-DENSITY-LIPOPROTEIN, AND ITS DELIVERY TO CULTURED HUMAN-FIBROBLASTS [J].

CANDIDE, C ;

MORLIERE, P ;

MAZIERE, JC ;

GOLDSTEIN, S ;

SANTUS, R ;

DUBERTRET, L ;

REYFTMANN, JP ;

POLONOVSKI, J .

FEBS LETTERS, 1986, 207 (01) :133-138

[6]

CARSON SD, 1987, J BIOL CHEM, V262, P718

[7]

EFFECTS OF LIPID-BINDING PROTEINS APO-A-I, APO-A-II, BETA-2-GLYCOPROTEIN-I, AND C-REACTIVE PROTEIN ON ACTIVATION OF FACTOR-X BY TISSUE FACTOR - FACTOR-VIIA [J].

CARSON, SD ;

ROSS, SE .

THROMBOSIS RESEARCH, 1988, 50 (05) :669-678

[8]

PLASMA HIGH-DENSITY LIPOPROTEINS INHIBIT THE ACTIVATION OF COAGULATION FACTOR-X BY FACTOR-VIIA AND TISSUE FACTOR [J].

CARSON, SD .

FEBS LETTERS, 1981, 132 (01) :37-40

[9]

CHEN GC, 1989, J BIOL CHEM, V264, P14369

[10]

REACTIVITY OF CHOLESTEROL AND SOME FATTY-ACIDS TOWARD SINGLET OXYGEN [J].

DOLEIDEN, FH ;

FAHRENHOLTZ, SR ;

LAMOLA, AA ;

TROZZOLO, AM .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1974, 20 (06) :519-521

← 1 2 3 4 →