INJECTION OF DIGITALLY SYNTHESIZED SYNAPTIC CONDUCTANCE TRANSIENTS TO MEASURE THE INTEGRATIVE PROPERTIES OF NEURONS

被引：169

作者：

ROBINSON, HPC ^{[1
]}

KAWAI, N ^{[1
]}

机构：

[1] JICHI MED SCH,DEPT PHYSIOL,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1993年 / 49卷 / 03期

关键词：

CONDUCTANCE INJECTION; SYNAPTIC CONDUCTANCE; CURRENT-CLAMP; HIPPOCAMPAL NEURON;

D O I：

10.1016/0165-0270(93)90119-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel technique was developed for injecting a time-varying conductance into a neuron, to allow quantitative measurement of the processing of synaptic inputs. In current-clamp recording mode, the membrane potential was sampled continuously and used to calculate and update the level of injected current within 60 mus, using a real-time computer, so as to mimic the electrical effect of a given conductance transient. Cellular responses to synthetic conductance transients modelled on the fast (non-N-methyl-D-aspartate) phase of the glutamatergic postsynaptic potential were measured in cultured rat hippocampal neurons.

引用

页码：157 / 165

页数：9

共 22 条

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