DETERMINATION OF THIAMINE AND ITS PHOSPHATE-ESTERS IN HUMAN ERYTHROCYTES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ISOCRATIC ELUTION

被引：30

作者：

HERVE, C ^{[1
]}

BEYNE, P ^{[1
]}

DELACOUX, E ^{[1
]}

机构：

[1] HOP BEAUJON,SERV BIOCHIM,100 BLVD GEN LECLERC,F-92110 CLICHY,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 653卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)E0438-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the simultaneous determination of thiamine and its phosphate esters in human erythrocytes, using postcolumn derivatization, is presented. The sample preparation and the choice of the analytical column avoid the use of an elution gradient. The four thiamine compounds (thiamine and thiamine monophosphate, diphosphate and triphosphate) are eluted within less than 15 min with a detection limit of ca. 20 fmol. The reproducibility and accuracy of the assay are satisfactory. Normal physiological red blood cell concentrations of the four thiamine compounds are included.

引用

页码：217 / 220

页数：4

共 17 条

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