MUTATIONS IN THE STRUCTURAL GENE FOR CYTOCHROME-C RESULT IN DEFICIENCY OF BOTH CYTOCHROME-AA(3) AND CYTOCHROME-C IN NEUROSPORA-CRASSA

The cyt-12-12 mutant of Neurospora crassa is characterized by slow growth and a deficiency of spectrophotometrically-detectable cytochromes aa(3) and c. Using a sib-selection procedure we have isolated the cyr-12(+) allele from a cosmid library of N. crassa genomic DNA. Characterization of the cyt-12(+) allele reveals that it encodes the structural gene for cytochrome c. DNA sequence analysis of the cyt-12-12 allele revealed a mutation in the cytochrome c coding sequence that results in replacement of a glycine residue, which is invariant in the cytochrome c of other species, with an aspartic acid. Genetic analysis confirms that cyt-12-12 is allelic with the previously-characterized cyc-1-1 mutant, which was also shown to affect the single locus encoding cytochrome c in N. crassa. We suggest that the amount of functional cytochrome c present in mitochondria influences the level of cytochrome aa(3).