PURIFICATION AND CHARACTERIZATION OF 2 EPOXIDE HYDROLASES FROM CORYNEBACTERIUM SP STRAIN-N-1074

被引：31

作者：

NAKAMURA, T

NAGASAWA, T

YU, FJ

WATANABE, I

YAMADA, H

机构：

[1] NAGOYA UNIV,DEPT APPL BIOL SCI,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

[2] KYOTO UNIV,DEPT AGR CHEM,SAKYO KU,KYOTO 606,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 12期

关键词：

D O I：

10.1128/AEM.60.12.4630-4633.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enzymes IIa and IIb, which catalyze the conversion of epichlorohydrin (ECH) to 3-chloro-1,2-propanediol (MCP), were purified from Corynebacterium sp. strain N-1074, which catalyzes the formation of (A)-MCP from prochiral 1,3-dichloro-2-propanol via ECH. The specific activity of enzyme IIa for the formation of MCP from ECH was about 6.4-fold higher than that of enzyme IIb. Both enzymes catalyzed the conversion of 1,2-epoxides to the corresponding diol, although they differed in several enzymatic properties.

引用

页码：4630 / 4633

页数：4

共 12 条

[1] ALLEN RH, 1969, J BIOL CHEM, V244, P2078
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] CHARACTERIZATION OF THE EPOXIDE HYDROLASE FROM AN EPICHLOROHYDRIN-DEGRADING PSEUDOMONAS-SP
JACOBS, MHJ
VANDENWIJNGAARD, AJ
PENTENGA, M
JANSSEN, DB
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (03): : 1217 - 1222
[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[5] HYDRATION OF 9,10-EPOXYPALMITIC ACID BY A SOLUBLE ENZYME FROM BACILLUS-MEGATERIUM
MICHAELS, BC
RUETTINGER, RT
FULCO, AJ
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 92 (04) : 1189 - 1195
[6] NAGASAWA T, 1992, APPL MICROBIOL BIOT, V36, P478
[7] RESOLUTION AND SOME PROPERTIES OF ENZYMES INVOLVED IN ENANTIOSELECTIVE TRANSFORMATION OF 1,3-DICHLORO-2-PROPANOL TO (R)-3-CHLORO-1,2-PROPANEDIOL BY CORYNEBACTERIUM SP STRAIN N-1074
NAKAMURA, T
NAGASAWA, T
YU, F
WATANABE, I
YAMADA, H
[J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (23) : 7613 - 7619
[8] PRODUCTION OF (R)-3-CHLORO-1,2-PROPANEDIOL FROM PROCHIRAL 1,3-DICHLORO-2-PROPANOL BY CORYNEBACTERIUM SP STRAIN N-1074
NAKAMURA, T
YU, FJ
MIZUNASHI, W
WATANABE, I
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (01) : 227 - 230
[9] MICROBIAL TRANSFORMATION OF PROCHIRAL 1,3-DICHLORO-2-PROPANOL INTO OPTICALLY-ACTIVE 3-CHLORO-1,2-PROPANEDIOL
NAKAMURA, T
YU, F
MIZUNASHI, W
WATANABE, I
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (07): : 1931 - 1933
[10] CHARACTERIZATION OF A NOVEL ENANTIOSELECTIVE HALOHYDRIN HYDROGEN-HALIDE-LYASE
NAKAMURA, T
NAGASAWA, T
YU, FJ
WATANABE, I
YAMADA, H
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (04) : 1297 - 1301

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