A MORPHOLOGICAL-STUDY ON GLUTAMATE-INDUCED SWELLING OF CULTURED ASTROCYTES - INVOLVEMENT OF CALCIUM AND CHLORIDE-ION MECHANISMS

被引：34

作者：

KOYAMA, Y

SUGIMOTO, T

SHIGENAGA, Y

BABA, A

IWATA, H

机构：

[1] OSAKA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,YAMADA OKA 1-6,SUITA,OSAKA 565,JAPAN

[2] OSAKA UNIV,FAC DENT,DEPT ORAL ANAT 2,SUITA,OSAKA 565,JAPAN

来源：

NEUROSCIENCE LETTERS | 1991年 / 124卷 / 02期

关键词：

L-GLUTAMATE; ASTROCYTE; SWELLING; CA2+; ULTRASTRUCTURE;

D O I：

10.1016/0304-3940(91)90102-Y

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Morphological changes in cultured astrocytes exposed to L-glutamate (Glu) were examined light and electron microscopically. The treatment with 0.1 mM Glu for 60 min caused marked swelling of the cells, which was characterized by reduction in staining of cytoplasm with Toluidine blue, disappearance of the cytoplasmic granular ground substances, swollen mitochondrion and nucleus, and dispersed chromatin. The above changes were prevented by the removal of Na+, Ca2+ or Cl- from the incubation medium for Glu treatment. However, the Glu treatment in a Cl- -free medium caused conspicuous aggregation of 10 nm filaments.

引用

页码：235 / 238

页数：4

共 19 条

[1] ELEVATION OF THE EXTRACELLULAR CONCENTRATIONS OF GLUTAMATE AND ASPARTATE IN RAT HIPPOCAMPUS DURING TRANSIENT CEREBRAL-ISCHEMIA MONITORED BY INTRACEREBRAL MICRODIALYSIS [J].