DETERMINATION OF PICOGRAM LEVELS OF BRAIN-SEROTONIN BY A SIMPLIFIED LIQUID-CHROMATOGRAPHIC ELECTROCHEMICAL DETECTION ASSAY

被引：20

作者：

WARSH, JJ ^{[1
]}

CHIU, A ^{[1
]}

GODSE, DD ^{[1
]}

COSCINA, DV ^{[1
]}

机构：

[1] UNIV TORONTO,CLARKE INST PSYCHIAT,BIOPSYCHOL SECT,TORONTO M5T 1R8,ONTARIO,CANADA

来源：

BRAIN RESEARCH BULLETIN | 1979年 / 4卷 / 04期

基金：

英国医学研究理事会;

关键词：

Brain areas; Electrochemical detection; High pressure liquid chromatography; Method; Rat; Serotonin;

D O I：

10.1016/0361-9230(79)90043-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A simplified assay is described for measurement of picogram amounts of serotonin (5-HT) by high pressure liquid chromatography with electrochemical detection (LCEC). The procedure involves pre-purification of brain 5-HT by adsorption on Amberlite CG-50. Serotonin is subsequently resolved and detected by LCEC on Zipax SCX resin. The present method gives working sensitivities of at least 100 pg, tissue recoveries of 95% and very low interassay variability (coefficient of variation=3%). Determination of rat brain area 5-HT by this LCEC method is described and compared to other high sensitivity methods. © 1979.

引用

页码：567 / 570

页数：4

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