DELETION OF CHROMOSOME-13 (BAND Q14) BUT NOT TRISOMY-12 IS A CLONAL EVENT IN B-CHRONIC LYMPHOCYTIC-LEUKEMIA (CLL)

被引：33

作者：

JABBAR, SAB ^{[1
]}

GANESHAGURU, K ^{[1
]}

WICKREMASINGHE, RG ^{[1
]}

HOFFBRAND, AV ^{[1
]}

FORONI, L ^{[1
]}

机构：

[1] ROYAL FREE HOSP,SCH MED,DEPT HAEMATOL,LONDON NW3 2QG,ENGLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1995年 / 90卷 / 02期

关键词：

CLL; DEL; 13Q14; TRISOMY; 12;

D O I：

10.1111/j.1365-2141.1995.tb05180.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Chromosomal abnormalities are detected by conventional cytogenetic or FISH analysis in 50% of chronic lymphocytic leukaemias (CLL). Trisomy 12 and del 13q14 account for 70% of these abnormalities. The incidence of these two abnormalities was studied in CLL patients by Southern blot analysis using a highly purified B-cell malignant population (CD5 > 95%, CD3 < 5%), Probes for the D13S25 marker on chromosome 13 band q14 and for the RBTN3 gene on chromosome 12 band p12-13, were used. Deletion of the D13S25 was detected in 17/42 patients (43%) in a homozygous (9.5%) or heterozygous (30%) configuration, Deletion of the D13S25 marker appears to be a clonal and early event in CLL development since it is detected in > 95% of the malignant clonal population, Conversely, trisomy 12 is rarely a clonal event (5/33 patients, 15%) and a varying proportion of cells carrying this abnormality can be demonstrated in 30% of CLL patients (10/33 patients).

引用

页码：476 / 478

页数：3

共 10 条

[1]

Bienz N., Cardy D.L.N., Hulten M.A., Trisomy 12 in B‐cell chronic lymphocytic leukaemia: an evaluation of 33 patients by direct fluorescence in situ hybridization (FISH), British Journal of Haematology, 85, pp. 819-822, (1993)

[2]

Brown A.G., Ross F.M., Eimer M.D., Steel CM., Weir-Thompson E.M., Evidence for a new tumour suppressor locus (DBM) in human B‐cell neoplasia telomeric to the retinoblastoma gene, Nature Genetics, 3, pp. 67-72, (1993)

[3]

Cannell P.K., Amlot P., Attard M., Hoffbrand A.V., Foroni L., Variable kappa gene rearrangement in lymphoprolifera‐tive disorders: an analysis of VK gene usage, VJ joining and somatic mutations, Leukemia, 8, pp. 1139-1145, (1994)

[4]

Chapman R.M., Corcoran M.M., Gardiner A., Hawthorn LA., Cowell J.K., Oscier D.G., Frequent homozygous deletion of the D13S25 locus in chromosome region 13ql4 defines the location of a gene critical in leukaemogenesis in chronic B cell lymphocytic leukaemia, Oncogene, 9, pp. 1289-1293, (1994)

[5]

Foroni L., Boehm T., White L., Forster A., Sherrington P.D., Rabbitts T.H., Three genes of rhombotin family encode highly related LIM‐domain proteins but are expressed differently in mouse development, Journal of Molecular Biology, 226, pp. 747-761, (1992)

[6]

Foroni L., Mason P.J., Luzzatto L., Ig and T cell receptor gene rearrangements, The Leukaemic Cell, pp. 339-391, (1991)

[7]

Hawthorn LA., Chapman R., Oscier D.G., Cowell J.K., The coexistent 13ql4 translocation breakpoint seen in chronic lymphocytic B‐cell leukaemia (B‐CLL) involves deletion of the D13S25 locus which lies distal to the retinoblastoma predisposition gene, Oncogene, 8, pp. 1415-1419, (1992)

[8]

Juliusson G., Oscier D.G., Fitchett M., Ross F.M., Stockdill G., Mackie M.J., Parker A.C., Castoldi G.L., Cuneo A., Knuutila S., Elonen E., Gahrton G., Prognostic subgroups in B‐cell chronic lymphocytic leukemia defined by specific chromosomal abnormalities, New England Journal of Medicine, 323, pp. 720-724, (1991)

[9]

Oscier D.G., Cytogenetic and molecular abnormalities in chronic lymphocytic leukaemia, Blood Reviews, 8, pp. 88-97, (1994)

[10]

Que T.H., Carcia Marco J., Ellis J., Matutes E., Brito-Babapulle V., Boyle S., Catovsky D., Trisomy 12 in chronic lymphocytic leukaemia detected by fluorescent in situ hybridisation by stage, immunophenotype and morphology, Blood, 82, pp. 571-575, (1993)

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