STUDIES ON THE PROCEDURE FOR THE CONSTRUCTION OF CELLULAR PROTEIN DATABASES EMPLOYING MICRO 2-DIMENSIONAL ELECTROPHORESIS - AN HL-60 PROTEIN DATABASE

被引：3

作者：

MANABE, T

YAMAMOTO, H

KAWAI, M

机构：

[1] HIMEJI INST TECHNOL, FAC SCI, DEPT MAT SCI, HIMEJI, HYOGO 67122, JAPAN

[2] AICHI MED UNIV, DEPT MICROBIOL, AICHI, JAPAN

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 03期

关键词：

CELLULAR PROTEINS; PROTEIN DATABASE; 2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; MICRO 2-DIMENSIONAL ELECTROPHORESIS; HL-60; PROTEINS;

D O I：

10.1002/elps.1150160168

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The procedure to construct a cellular protein database employing a micro two-dimensional electrophoresis (2-DE) technique was studied. Protein information on human cultured leukemic cell HL-60 was collected as an HL-60 protein database, which consists of a 2-DE polypeptide map of 670 recognized spots with spot numbering and information on isoelectric point (pI), molecular mass, and percent integrated density. The procedure employed in this report will be applicable for the comparative studies of cellular proteins and will help to construct comprehensive cellular protein databases.

引用

页码：407 / 422

页数：16

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