PLAQUE ASSAY OF BACULOVIRUSES EMPLOYING AN AGAROSE-NUTRIENT OVERLAY

被引：16

作者：

KNUDSON, DL

机构：

来源：

INTERVIROLOGY | 1979年 / 11卷 / 01期

关键词：

D O I：

10.1159/000149010

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Four baculoviruses produced visible plaques in the Spodoptera frugiperda cell line when cell monolayers were infected and overlayed with a simplified, agarose-nutrient formulation. Macroscopic plaques were first detected 4 days postinoculation, and by 10 days plaques ranging from 0.5 to 3 mm in diameter were seen. Dose-response experiments indicated that a single particle initiated the formation of plaque, because a linear response was demonstrated with increased dosage.

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页码：40 / 46

页数：7

相关论文

共 13 条

[1] PLAQUE ASSAY FOR NUCLEAR POLYHEDROSIS VIRUSES USING A SOLID OVERLAY [J].

BROWN, M ;

FAULKNER, P .

JOURNAL OF GENERAL VIROLOGY, 1977, 36 (AUG) :361-364

[2] INTERPRETATION OF HOST RESPONSE IN QUANTITATIVE STUDIES ON ANIMAL VIRUSES [J].

BRYAN, WR .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1957, 69 (04) :698-728

[3] 2 TISSUE-CULTURE MEDIA FOR PRODUCTION OF LEPIDOPTERAN CELLS AND NUCLEAR POLYHEDROSIS VIRUSES [J].

GARDINER, GR ;

STOCKDALE, H .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1975, 25 (03) :363-370

[4]

Granados R R, 1976, Adv Virus Res, V20, P189, DOI 10.1016/S0065-3527(08)60505-9

[5] PROPERTIES OF 3 BACULOVIRUSES FROM CLOSELY RELATED HOSTS [J].

HARRAP, KA ;

PAYNE, CC ;

ROBERTSON, JS .

VIROLOGY, 1977, 79 (01) :14-31

[6] IMPROVED TECHNIQUE FOR PLAQUE ASSAY OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS ON TN-368 CELLS [J].

HINK, WF ;

STRAUSS, EM .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1977, 29 (03) :390-391

[7] PLAQUE ASSAY FOR TITRATION OF ALFALFA LOOPER NUCLEAR POLYHEDROSIS-VIRUS IN A CABBAGE-LOOPER (TN-368) CELL LINE [J].

HINK, WF ;

VAIL, PV .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1973, 22 (02) :168-174

[8] DNA CONTAINED BY NUCLEAR POLYHEDROSIS VIRUSES ISOLATED FROM 4 SPODOPTERA SP (LEPIDOPTERA, NOCTUIDAE) - GENOME SIZE AND HOMOLOGY ASSESSED BY DNA REASSOCIATION KINETICS [J].

KELLY, DC .

VIROLOGY, 1977, 76 (01) :468-471

[9] REPLICATION OF A NUCLEAR POLYHEDROSIS-VIRUS IN A CONTINUOUS CELL-CULTURE OF SPODOPTERA-FRUGIPERDA - PURIFICATION, ASSAY OF INFECTIVITY, AND GROWTH-CHARACTERISTICS OF VIRUS [J].

KNUDSON, DL ;

TINSLEY, TW .

JOURNAL OF VIROLOGY, 1974, 14 (04) :934-944

[10] REPLICATION OF A NUCLEAR POLYHEDROSIS-VIRUS IN A CONTINUOUS CELL-CULTURE OF SPODOPTERA-FRUGIPERDA - MICROSCOPY STUDY OF SEQUENCE OF EVENTS OF VIRUS-INFECTION [J].

KNUDSON, DL ;

HARRAP, KA .

JOURNAL OF VIROLOGY, 1976, 17 (01) :254-268