INHIBITION OF CYTOCHALASIN D-STIMULATED G-ACTIN ATPASE BY ADP-RIBOSYLATION WITH CLOSTRIDIUM-PERFRINGENS IOTA TOXIN

被引：25

作者：

GEIPEL, U ^{[1
]}

JUST, I ^{[1
]}

AKTORIES, K ^{[1
]}

机构：

[1] UNIV GIESSEN,RUDOLF BUCHHEIM INST PHARMAKOL,FRANKFURTERSTR 107,W-6300 GIESSEN,GERMANY

来源：

BIOCHEMICAL JOURNAL | 1990年 / 266卷 / 02期

关键词：

D O I：

10.1042/bj2660335

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Clostridium perfringens iota toxin belongs to a novel family of actin-ADP-ribosylating toxins. The effects of ADP-ribosylation of skeletal muscle actin by Clostridium perfringens iota toxin on cytochalasin D-stimulated acin ATPase activity was studied. Cytochalasin D stimulated actin-catalysed ATP hydrolysis maximally by about 30-fold. ADP-ribosylation of actin completely inhibited cytochalasin D-stimulated ATP hydrolysis. Inhibition of ATPase activity occurred at actin concentrations below the critical concentration (0.1 μM), at low concentrations of Mg2+ (50 μM) and even in the actin-DNAase I complex, indicating that ADP-ribosylation of actin blocks the ATPase activity of monomeric actin and that the inhibitory effect is not due to inhibition of the polymerization of actin.

引用

页码：335 / 339

页数：5

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