AUTOMATION OF ATRAZINE AND ALACHLOR EXTRACTION FROM SOIL USING A LABORATORY ROBOTIC SYSTEM

被引:43
作者
KOSKINEN, WC
JARVIS, LJ
DOWDY, RH
WYSE, DL
BUHLER, DD
机构
[1] UNIV MINNESOTA,DEPT SOIL SCI,ST PAUL,MN 55108
[2] UNIV MINNESOTA,DEPT AGRON & PLANT GENET,ST PAUL,MN 55108
[3] USDA ARS,PLANT SCI RES UNIT,ST PAUL,MN 55108
关键词
D O I
10.2136/sssaj1991.03615995005500020047x
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
In order to analyze our ever-increasing number of samples, we developed an automated multistep procedure for the extraction of atrazine (6-chloro-N-ethyl-N'-[1-methyl ethyl]-1,3,5-triazine-2,4-diamine) and alachlor (2-chloro-N-[2,6-diethylphenyl]-N-[methoxymethyl] acetamide) from soil using a commercially available laboratory robotic system. The robotic system shakes the soil-extraction solvent mixture, separates solvent from soil, evaporates methanol from the solvent, performs a liquid-solid extraction on the remaining herbicide-water solution, transfers the eluate containing the herbicides and internal standard to a gas chromatograph (GC) vial, and caps the vial. Serial robotic processing of samples, compared with manual batch processing, increased sample throughout by a factor of three and decreased labor by 50%. Efficiency and precision of extraction of atrazine and alachlor increased to 89 +/- 2% (robotic) from 79 +/- 17% (manual). At present, the soil weight of 10 g results in a minimum detectable amount of 10-mu-g herbicide kg-1 soil.
引用
收藏
页码:561 / 562
页数:2
相关论文
共 4 条
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