THE LAC REPRESSOR AND ITS N-TERMINAL HEADPIECE CAN BIND A MINI-OPERATOR CONTAINING A HAIRPIN LOOP MADE OF A HEXAETHYLENE GLYCOL CHAIN

被引：4

作者：

MAURIZOT, JC

CHEVRIE, K

DURAND, M

THUONG, NT

机构：

来源：

FEBS LETTERS | 1991年 / 288卷 / 1-2期

关键词：

LAC REPRESSOR; HEADPIECE; MODIFIED OLIGONUCLEOTIDE; HAIRPIN; DNA-PROTEIN INTERACTION;

D O I：

10.1016/0014-5793(91)81012-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of the lac repressor and the lac repressor N-terminal headpiece to a mini-operator with a hairpin loop made of a hexaethylene glycol chain was investigated using circular dichroism spectroscopy. The lac repressor's headpiece binds to the modified mini-operator with the same affinity as to a mini-operator of the same sequence without the hexaethylene glycol loop. The conformatoinal effect due to the binding is not affected by the presence of the hexaethylene loop. It is also shown that the entire lac repressor binds to this modified mini-operator inducing a conformational change.

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页码：101 / 104

页数：4

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