SDS-PRESERVATION FOR DEFERRED MEASUREMENT OF EXOPROTEOLYTIC KINETICS IN MARINE SAMPLES

被引：11

作者：

DELMAS, D

GARET, MJ

机构：

[1] Crema l'Houmeau (CNRS/IFREMER), 17137 l'Houmeau

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1995年 / 22卷 / 03期

关键词：

BACTERIAL EXOPROTEOLYTIC ACTIVITY; SODIUM DODECYL SULFATE INHIBITION; MARINE SAMPLE STORAGE; DEFERRED ANALYSIS;

D O I：

10.1016/0167-7012(95)00008-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of sodium dodecyl sulfate (SDS) on exoproteolytic activity was studied in order to test its efficacy as a preservative during deferred analysis of incubated samples. Exoproteolytic activities were assayed using fluorogenic leucine-methylcoumarinyl amide as substrate. Addition of SDS at 1% final concentration entirely inhibited proteolytic activity for both marine pond water and sediment samples. The kinetic parameters, V-m and K-m, of SDS-inhibited samples did not significantly alter during storage at -20 degrees C. The possibility to preserve incubated samples is already proving very useful in field studies where large numbers of exoproteolytic activity measurements cannot be performed immediately.

引用

页码：243 / 248

页数：6

共 14 条

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