IMMUNOHISTOCHEMICAL DEMONSTRATION OF CELL-SURFACE ANTIGENS ON TISSUE-SECTIONS OF LYMPHOMAS

被引：16

作者：

HOFFMANNFEZER, G ^{[1
]}

THIERFELDER, S ^{[1
]}

PIELSTICKER, K ^{[1
]}

RODT, H ^{[1
]}

机构：

[1] UNIV MUNICH,INST PATHOL,D-8000 MUNICH 2,FED REP GER

来源：

LEUKEMIA RESEARCH | 1979年 / 3卷 / 05期

关键词：

ALL; Immunohistochemistry; Lymphoma; Surface antigens;

D O I：

10.1016/0145-2126(79)90056-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

An immunohistochemical technique using the unlabeled antibody enzyme method was applied to tissue sections for identification of cells according to their surface antigens. The method and its diagnostic possibilities were demonstrated on four types of malignant lymphomas. Follicular centroblastic-centrocytic lymphomas contain T-cell regions distinctly separated from the follicular areas containing B-lymphocytes staining for one immunoglobulin class only. Lymphoplasmacytoid lymphomas have a small number of T-lymphocytes distributed between tumour cells as single cells or as small clusters. Whereas the majority of tumour cells showed ringlike labeling with anti-immunoglobulin, a small number of cells revealed diffuse cytoplasmic Ig-staining. Two cases with tumorous infiltrations of non-lymphatic tissues could be ascribed to ALL because they contained tumour cells stained with AcALLG. In one of them perivascular tumour cells positive with both AcALLG and anti-IgM were classified as pre-B cells. © 1979.

引用

页码：297 / 304

页数：8

共 14 条

[1] BACKGROUND STAINING AND SENSITIVITY OF UNLABELLED ANTIBODY-ENZYME (PAP) METHOD - COMPARISON WITH PEROXIDASE LABELED ANTIBODY SANDWICH METHOD USING FORMALIN FIXED PARAFFIN EMBEDDED MATERIAL [J].

BURNS, J .

HISTOCHEMISTRY, 1975, 43 (03) :291-294

[2]

GERARDMA.R, 1974, LANCET, V2, P406

[3]

Hoffmann-Fezer G, 1978, Verh Dtsch Ges Pathol, V62, P371

[4] ANATOMICAL DISTRIBUTION OF T AND B LYMPHOCYTES IDENTIFIED BY IMMUNOHISTOCHEMISTRY IN CHICKEN SPLEEN [J].

HOFFMANNFEZER, G ;

RODT, H ;

GOTZE, D ;

THIERFELDER, S .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1977, 55 (1-6) :86-95

[5] IMMUNOHISTOCHEMICAL IDENTIFICATION OF T-LYMPHOCYTES AND B-LYMPHOCYTES DELINEATED BY UNLABELED ANTIBODY ENZYME METHOD .1. ATOMICAL DISTRIBUTION OF THETA-POSITIVE AND IG-POSITIVE CELLS IN LYMPHOID ORGANS OF MICE [J].

HOFFMANNFEZER, G ;

RODT, H ;

EULITZ, M ;

THIERFELDER, S .

JOURNAL OF IMMUNOLOGICAL METHODS, 1976, 13 (3-4) :261-270

[6]

KAISERLING E, 1977, NON HODGKIN LYMPHOME, P30

[7]

LUKES RJ, 1974, CANCER, V34, P1488, DOI 10.1002/1097-0142(197410)34:8+<1488::AID-CNCR2820340822>3.0.CO

[8]

2-C

[9] ALL-ASSOCIATED ANTIGEN - OCCURRENCE ON NORMAL BLOOD-CELLS, CELL-LINES AND LEUKEMIC-CELLS [J].

RODT, H ;

NETZEL, B ;

THIEL, E ;

HAAS, RJ ;

HUHN, D ;

THIERFELDER, S .

BLUT, 1978, 36 (06) :339-345

[10] IDENTIFICATION AND QUANTITATION OF HUMAN T-CELL ANTIGEN BY ANTISERA PURIFIED FROM ANTIBODIES CROSSREACTING WITH HEMATOPOIETIC PROGENITORS AND OTHER BLOOD-CELLS [J].

RODT, H ;

THIERFELDER, S ;

THIEL, E ;

GOTZE, D ;

NETZEL, B ;

HUHN, D ;

EULITZ, M .

IMMUNOGENETICS, 1975, 2 (05) :411-430

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