ENHANCED CHEMILUMINESCENCE FOR TISSUE ANTIGEN AND CELLULAR VIRAL-DNA DETECTION

被引：13

作者：

HAWKINS, E

CUMMING, R

机构：

[1] Corporate Research, Amersham International, Little Chalfont, Bucks., Pollards Wood

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1990年 / 38卷 / 03期

关键词：

Enhanced chemiluminescence; Human Bladder carcinoma cells; Human CaSki cells; Human colon tissue; Immunocytochemistry; In situ hybridization;

D O I：

10.1177/38.3.1689340

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The ability to use enhanced chemiluminescence (ECL) to detect horseradish peroxidase as a label for tissue antigens and cellular viral DNA was demonstrated. A liquid nitrogen-cooled charged-coupled device (CCD) was used to detect light output, which was visualized on a monitor or was quantitated using an attached microcomputer. In a tissue antigen model, equivalent sensitivity was observed between ECL and colorimetric detection.

引用

页码：415 / 419

页数：5