EFFICIENT TRANSFECTION OF MOSQUITO CELLS IS INFLUENCED BY THE TEMPERATURE AT WHICH DNA-CALCIUM PHOSPHATE COPRECIPITATES ARE PREPARED

被引：10

作者：

KJER, KM

FALLON, AM

机构：

[1] Department of Entomology, University of Minnesota, St. Paul, Minnesota

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 1991年 / 16卷 / 03期

关键词：

CULTURED INSECT CELLS; AEDES-ALBOPICTUS; TRANSIENT EXPRESSION; HSP-CAT PLASMID;

D O I：

10.1002/arch.940160305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transient expression of a heat-shock protein-chloramphenicol acetyltrans-Perase (hsp-CAT) recombinant plasmid was used to define the parameters that influence transfection of cultured mosquito cells using DNA-calcium phosphate coprecipitates. The efficiency of the calcium phosphate procedure was strongly influenced by the growth state of recipient cells, and by the temperature at which the coprecipitate was prepared. Under optimal conditions, which included formation of the DNA-calcium phosphate coprecipitate at 50-degrees-C, transfection frequencies were up to tenfold higher than those obtained using the previously described polybrene procedure.

引用

页码：189 / 200

页数：12

共 32 条

[1] CLONED DROSOPHILA ALCOHOL-DEHYDROGENASE GENES ARE CORRECTLY EXPRESSED AFTER TRANSFECTION INTO DROSOPHILA CELLS IN CULTURE [J].