USE OF IMMOBILIZED METAL-IONS AS A NEGATIVE ADSORBENT FOR PURIFICATION OF ENZYMES - APPLICATION TO PHOSPHOGLYCERATE MUTASE FROM CHICKEN MUSCLE EXTRACT AND HORSERADISH-PEROXIDASE

被引：8

作者：

CHAGA, G ^{[1
]}

ANDERSSON, L ^{[1
]}

ERSSON, B ^{[1
]}

BERG, M ^{[1
]}

机构：

[1] INST BIOPROD,BU-4003 PLOVDIV,BULGARIA

来源：

BIOMEDICAL CHROMATOGRAPHY | 1992年 / 6卷 / 04期

关键词：

D O I：

10.1002/bmc.1130060404

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two enzymes, phosphoglycerate mutase and peroxidase, were purified by using an immobilized metal ion adsorbent for the removal of unwanted proteins. The mutase was obtained pure from a single column, whereas the purification of peroxidase required the use of a thiophilic adsorbent in a tandem. The capacity was 2.5 mg pure peroxidase per mL gel.

引用

收藏

页码：172 / 176

页数：5

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