A COMPLETE CDNA FOR ADENINE PHOSPHORIBOSYLTRANSFERASE FROM ARABIDOPSIS-THALIANA

被引：27

作者：

MOFFATT, BA

MCWHINNIE, EA

BURKHART, WE

PASTERNAK, JJ

ROTHSTEIN, SJ

机构：

[1] GLAXO RES LABS, MORRISVILLE, NC 27560 USA

[2] UNIV GUELPH, DEPT MOLEC BIOL & GENET, GUELPH N1G 2W1, ONTARIO, CANADA

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 04期

关键词：

ADENINE PHOSPHORIBOSYLTRANSFERASE; ARABIDOPSIS-THALIANA; PURINE METABOLISM; SELECTABLE MARKER;

D O I：

10.1007/BF00020008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An intact cDNA from Arabidopsis thaliana for adenine phosphoribosyltransferase (APRT) was isolated and sequenced. The cDNA is 729 nucleotides in length and predicts a protein of M(r) 27 140. The deduced amino acid sequence has been compared with those of other APRTs and shown to be most similar to the Escherichia coli protein. Construction of a molecular tree of the known APRT amino acid sequences indicates the A. thaliana and E. coli APRT sequences form one cluster and the currently available vertebrate and invertebrate sequences form a separate grouping. Since it is possible to select either for or against the expression of APRT, the isolation of this APRT cDNA clone will allow these selection schemes to be used in plant genetic experiments.

引用

页码：653 / 662

页数：10

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