ULTRASTRUCTURE OF MICROSPOROGENESIS AND MICROGAMETOGENESIS IN ARABIDOPSIS-THALIANA (L) HEYNH ECOTYPE WASSILEWSKIJA (BRASSICACEAE)

被引:261
作者
OWEN, HA [1 ]
MAKAROFF, CA [1 ]
机构
[1] MIAMI UNIV, DEPT CHEM, OXFORD, OH 45056 USA
关键词
ARABIDOPSIS THALIANA; MICROGAMETOGENESIS; POLLEN DEVELOPMENT; TAPETAL CELLS; ULTRASTRUCTURE;
D O I
10.1007/BF01272749
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The process of microsporogenesis and microgametogenesis was studied at the ultrastructural level in wild-type Arabidopsis thaliana ecotype Wassilewskija to provide a basis for comparison with nuclear male-sterile mutants of the same ecotype. From the earliest stage studied to mature pollen just prior to anther dehiscence, microsporocyte/microspore/pollen development follows the general pattern seen in most angiosperms. The tapetum is of the secretory type with loss of the tapetal cell walls beginning at about the time of microsporocyte meiosis. Wall loss exhibits polarity with the tapetal protoplasts becoming located at a distance from the inner tangential walls first, followed by an increase in distance from the radial walls beginning al the interior edge and progressing outward. The inner tangential and radial tapetal walls are completely degenerated by the microspore tetrad stage. Unlike other members of the Brassicaceae that have been studied, the tapetal cells of A. thaliana Wassilewskija also lose their outer tangential walls, and secretion occurs from all sides of the cells. Exine wall precursors are secreted from the tapetal cells in a process that appears to involve dilation of individual endoplasmic reticulum cisternae that fuse with the tapetal cell membrane and release their contents into the locule. Following completion of the exine, the tapetal cell plastids develop membrane-bound inclusions with osmiophilic and electron-transparent regions. The plastids undergo ultrastructural changes that suggest breakdown of the inclusion membranes followed by release of their contents into the locule prior to the complete degeneration of the tapetal cells.
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页码:7 / 21
页数:15
相关论文
共 23 条
[1]  
[Anonymous], 1974, POLLEN
[2]   ORIGIN OF SPERM CELL ASSOCIATION IN THE MALE GERM UNIT OF BRASSICA POLLEN [J].
CHARZYNSKA, M ;
MURGIA, M ;
MILANESI, C ;
CRESTI, M .
PROTOPLASMA, 1989, 149 (01) :1-4
[3]  
DAHL OA, 1986, POLLEN SPORES FORM F, P49
[4]   MICROSPORE AND POLLEN DEVELOPMENT IN 6 MALE-STERILE MUTANTS OF ARABIDOPSIS-THALIANA [J].
DAWSON, J ;
WILSON, ZA ;
AARTS, MGM ;
BRAITHWAITE, AF ;
BRIARTY, LG ;
MULLIGAN, BJ .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1993, 71 (04) :629-638
[5]   FORMATION OF TRYPHINE COATING POLLEN GRAINS OF RAPHANUS, AND ITS PROPERTIES RELATING TO SELF-INCOMPATIBILITY SYSTEM [J].
DICKINSON, HG ;
LEWIS, D .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1973, 184 (1075) :149-+
[6]  
DICKINSON HG, 1973, CYTOBIOS, V8, P25
[7]  
Feldmann K. A., 1992, Methods in Arabidopsis research., P274
[8]   T-DNA INSERTION MUTAGENESIS IN ARABIDOPSIS - MUTATIONAL SPECTRUM [J].
FELDMANN, KA .
PLANT JOURNAL, 1991, 1 (01) :71-82
[9]   A COMPARATIVE LIGHT AND ELECTRON-MICROSCOPIC STUDY OF MICROSPORE AND TAPETAL DEVELOPMENT IN MALE FERTILE AND CYTOPLASMIC MALE STERILE OILSEED RAPE (BRASSICA-NAPUS) [J].
GRANT, I ;
BEVERSDORF, WD ;
PETERSON, RL .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1986, 64 (05) :1055-1068
[10]   POLYCHROMATIC STAINS FOR THIN SECTIONS OF BETA EMBEDDED IN EPOXY RESIN [J].
HOEFERT, LL .
STAIN TECHNOLOGY, 1968, 43 (03) :145-&