DETECTION OF BOVINE GROUP-B ROTAVIRUSES IN FECES BY POLYMERASE CHAIN-REACTION

被引：16

作者：

CHINSANGARAM, J

AKITA, GY

OSBURN, BI

机构：

[1] Department of Veterinary Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1994年 / 6卷 / 03期

关键词：

D O I：

10.1177/104063879400600304

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A pair of primers designed from the sequence of genome segment 9 of group B rat rotavirus (IDIR) were employed to amplify genome segment 9 of a group B bovine rotavirus in a polymerase chain reaction (PCR) and to sequence the derived PCR products. A new pair of primers were synthesized from the obtained sequence data and used in a PCR detection assay for group B bovine rotavirus in fecal samples. In addition, another pair of primers were designed to produce a PCR-derived internal probe. This probe was used in a chemiluminescent hybridization to confirm the specificity and to increase the sensitivity of the assay. This assay could detect 0.1 fg of target double-stranded RNA. It was specific to group B bovine rotavirus and did not detect group B rat (IDIR) and porcine rotaviruses, group A bovine (NCDV), simian (SA-11), equine (H-2), porcine (OSU), human (DS-1), deer, and avian rotaviruses, coronavirus, or other enteric organisms tested in this study.

引用

页码：302 / 307

页数：6

共 21 条

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