PYRIMIDINE DEOXYRIBONUCLEOSIDE PHOSPHORYLASE .I. SUBSTRATE INHIBITION BY THYMINE

被引：6

作者：

LYON, GM

机构：

[1] Department of Pediatrics, Duke University Medical Center, Durham, NC

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 159卷 / 01期

关键词：

D O I：

10.1016/0005-2744(68)90242-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thymidine phosphorylase (thymidine:orthophosphate deoxyribosyltranferase, EC 2.4.2.4) has been approximately 500-fold purified from adult rabbit liver and the kinetics of the conversion of thymine to thymidine, with deoxyribose-i-phosphate as deoxyribosyl donor, are presented. The reaction is subject to inhibition by high concentrations of thymine and this inhibition is modified by urea or low pH. The inhibition persists when the reaction is carried out in 0.5 M NaCl. The effect of both thymine and urea are immediately reversible, and the inhibition by thymine is non-competitive with respect to deoxyribosei-phosphate. © 1968.

引用

页码：38 / &

共 19 条

[1] FEEDBACK INHIBITION OF URIDINE KINASE BY CYTIDINE TRIPHOSPHATE + URIDINE TRIPHOSPHATE [J].

ANDERSON, EP ;

BROCKMAN, RW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 91 (03) :380-&

[2]

ATKINSON DE, 1965, J BIOL CHEM, V240, P2682

[3]

COLOWICK SP, 1955, METHOD ENZYMOL, V1, P76

[4]

DIXON M, 1964, ENZYMES, P78

[5]

FINK K, 1956, J BIOL CHEM, V221, P425

[6]

FLORKIN M, 1963, COMP BIOCHEM, V6, P73

[7]

FRIEDKIN M, 1954, J BIOL CHEM, V207, P245

[8]

GALLO RC, 1967, J BIOL CHEM, V242, P5059

[9]

GREEN AA, 1955, METHOD ENZYMOL, V1, P76

[10]

HOTCHKISS RD, 1948, J BIOL CHEM, V175, P315

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