ACTIVATION OF CYCLIC AMP-DEPENDENT PROTEIN-KINASES BY VASOACTIVE INTESTINAL PEPTIDE (VIP) IN ISOLATED INTESTINAL EPITHELIAL-CELLS FROM RAT

被引：34

作者：

LABURTHE, M

MANGEAT, P

MARCHISMOUREN, G

ROSSELIN, G

机构：

[1] Unite de Recherche de Diabetologie et d'Etudes Radio-Immunologiques des Hormones Proteiques (ERA 494, hôpital Saint-Antoine

[2] Institut de Chimie Biologique (LA 202, CNRS), Université d'Aix-Marseille, 13331 Marseille Cedex 3, Place Victor Hugo

来源：

LIFE SCIENCES | 1979年 / 25卷 / 22期

关键词：

D O I：

10.1016/0024-3205(79)90615-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

VIP stimulates protein kinase activity in intestinal epithelial cells isolated from rat jejuno-ileum. The stimulation is time-dependent and is potentiated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. The response occurs in the 0.1-10 nM range of VIP concentrations, half-maximal stimulation being observed with 0.7 nM VIP. The VIP-induced protien kinase activation is thus observed at concentrations similar to those promoting the accumulation of cyclic AMP (11). Secretin also stimulates protien kinase activity but with a 100-times lower potency than VIP, in agreement with the fact that secretin is a VIP agonist of 100-times lower potency with respect to cyclic AMP increase. Prostaglandins E1 and E2 (10-5 M), are also found to increase protein kinase activity. © 1979.

引用

页码：1931 / 1938

页数：8

共 23 条

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