SYNTHESIS, SPECTRAL PROPERTIES AND ENZYMATIC-HYDROLYSIS OF FLUORESCENT DERIVATIVES OF CEREBROSIDE SULFATE CONTAINING LONG-WAVELENGTH-EMISSION PROBES

被引：28

作者：

MARCHESINI, S ^{[1
]}

PRETI, A ^{[1
]}

ALEO, MF ^{[1
]}

CASELLA, A ^{[1
]}

DAGAN, A ^{[1
]}

GATT, S ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT MEMBRANE BIOCHEM & NEUROCHEM,IL-91010 JERUSALEM,ISRAEL

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1990年 / 53卷 / 2-3期

关键词：

arylsulfatase A; fluorescent sulfatides; long-wavelength-emission fluorophores;

D O I：

10.1016/0009-3084(90)90042-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent derivatives of cerebroside sulfate (sulfogalactosyl ceramide, sulfatide) containing long-wavelength-emission fluorophores were synthesized. For this purpose a procedure was developed for preparing a cerebroside 3-sulfate derivative with an amino group on the terminal carbon atom of its fatty acyl residue. The latter compound has been used to prepare cerebroside 3-sulfate, coupled to lissamine-rhodamine, fluoresceine, eosine and NBD. The spectroscopic properties of these compounds, in different solvent systems and when incorporated into micelles of a non-ionic detergent or liposomes of a phospholipid, are reported. Incubation of these respective sulfatides with a human leukocyte preparation, resulted in the formation of the corresponding fluorescent cerebrosides. © 1990.

引用

页码：165 / 175

页数：11

共 15 条

[1] STEAROYL[1-C-14]SULFOGALACTOSYLSPHINGOSINE ([C-14]SULFATIDE) AS SUBSTRATE FOR CEREBROSIDE SULFATASE ASSAY [J].