RECOVERY OF DNA FROM THE ACINETOBACTER-CALCOACETICUS CHROMOSOME BY GAP REPAIR

被引：26

作者：

GREGGJOLLY, LA ^{[1
]}

ORNSTON, LN ^{[1
]}

机构：

[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06511

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 10期

关键词：

D O I：

10.1128/jb.172.10.6169-6172.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A strain of Acinetobacter calcoaceticus that demonstrates unusually high competence for natural transformation by linear DNA has proven valuable for analysis of genes and gene clusters associated with aromatic catabolism. The transformation system allowed gap repair to be used to recover mutant chromosomal DNA within recombinant plasmids. The sizes of the recovered fragments, 5 and 7 kilobase pairs in length, indicate that gap repair will be a useful procedure for isolation of wile-type and modified gene clusters from the A. calcoaceticus chromosome.

引用

页码：6169 / 6172

页数：4

共 21 条

[1] CANAVAS JL, 1968, EUR J BIOCHEM, V3, P293
[2] INFLUENCE OF THE CATBCE SEQUENCE ON THE PHENOTYPIC REVERSION OF A PCAE MUTATION IN ACINETOBACTER-CALCOACETICUS
DOTEN, RC
GREGG, LA
ORNSTON, LN
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (07) : 3175 - 3180
[3] CLONING AND GENETIC ORGANIZATION OF THE PCA GENE-CLUSTER FROM ACINETOBACTER-CALCOACETICUS
DOTEN, RC
NGAI, KL
MITCHELL, DJ
ORNSTON, LN
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (07) : 3168 - 3174
[4] DNA-SEQUENCES OF GENES ENCODING ACINETOBACTER-CALCOACETICUS PROTOCATECHUATE 3,4-DIOXYGENASE - EVIDENCE INDICATING SHUFFLING OF GENES AND OF DNA-SEQUENCES WITHIN GENES DURING THEIR EVOLUTIONARY DIVERGENCE
HARTNETT, C
NEIDLE, EL
NGAI, KL
ORNSTON, LN
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (02) : 956 - 966
[5] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS
HOLMES, DS
QUIGLEY, M
[J]. ANALYTICAL BIOCHEMISTRY, 1981, 114 (01) : 193 - 197
[6] RAPID AND EFFICIENT COSMID CLONING
ISHHOROWICZ, D
BURKE, JF
[J]. NUCLEIC ACIDS RESEARCH, 1981, 9 (13) : 2989 - 2998
[7] TRANSFORMATION OF ACINETOBACTER CALCO-ACETICUS (BACTERIUM ANITRATUM)
JUNI, E
JANIK, A
[J]. JOURNAL OF BACTERIOLOGY, 1969, 98 (01) : 281 - +
[8] IMPROVED BROAD-HOST-RANGE PLASMIDS FOR DNA CLONING IN GRAM-NEGATIVE BACTERIA
KEEN, NT
TAMAKI, S
KOBAYASHI, D
TROLLINGER, D
[J]. GENE, 1988, 70 (01) : 191 - 197
[9] KOBAYASHI I, 1988, GENETICS, V119, P751
[10] DOUBLE-STRANDED GAP REPAIR IN THE PHOTOSYNTHETIC PROKARYOTE SYNECHOCOCCUS-R2
KOLOWSKY, KS
SZALAY, AA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (15) : 5578 - 5582

← 1 2 3 →