FLUORESCENCE QUANTUM YIELD DETERMINATION BY PULSED-SOURCE SINGLE PHOTON-COUNTING

被引：10

作者：

UPTON, LM ^{[1
]}

LOVE, LJC ^{[1
]}

机构：

[1] SETON HALL UNIV, DEPT CHEM, S ORANGE, NJ 07079 USA

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 12期

关键词：

D O I：

10.1021/ac50048a012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method of measuring fluorescence quantum yields by using time correlated single photon counting instrumentation was developed. The principles of single photon counting by using pulsed excitation light sources for quantitative measurements of absorbance and fluorescence intensity are presented. The single photon count rate was found to be proportional to the light flux up to a frequency of about 10% of the pulsed-light-source, 25-kHz repetition rate. Experiment efficiency rates greater than 10% produced multiple photon detection and pulse pileup errors. The quantum yields agreed within ±0.05 with previously published values and those made by the conventional integration method. The expression for the minimum relative error in the quantum yield due to counting statistics is presented. The actual precision was ±0.001 and was determined by drift in the lamp intensity. © 1979, American Chemical Society. All rights reserved.

引用

页码：1941 / 1945

页数：5

共 21 条

[1] METHODS FOR DETECTING WEAK LIGHT SIGNALS [J].