LIPOPOLYAMINE-MEDIATED TRANSFECTION ALLOWS GENE-EXPRESSION STUDIES IN PRIMARY NEURONAL CELLS

被引：53

作者：

LOEFFLER, JP

BARTHEL, F

FELTZ, P

BEHR, JP

SASSONECORSI, P

FELTZ, A

机构：

[1] INST LE BEL,CHIM ORGAN LAB,CNRS,U 422,STRASBOURG,FRANCE

[2] FAC MED STRASBOURG,CNRS,GENET MOLEC EUCARYOTES LAB,STRASBOURG,FRANCE

[3] FAC MED STRASBOURG,INST CHIM BIOL,INSERM,U184,STRASBOURG,FRANCE

[4] LAB ETUD REGULAT PHYSIOL,STRASBOURG,FRANCE

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 05期

关键词：

12‐O‐Tetradecanoylphorbol 13‐acetate‐inducible elements; Cerebellum; Cyclic AMP‐inducible elements; Granular cells; Primary neurons; Trans‐acting factors;

D O I：

10.1111/j.1471-4159.1990.tb01240.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: A simple and efficient transfection technique based on lipopolyamine‐coated DNA that can be used for gene transfer in cerebellar granular neurons is described. Gene transfer is achieved by exposure of cells to a DNA/lipid complex obtained by simple mixing of lipopolyamine and plasmid DNA. This procedure may represent a general tool of physiological investigations in primary cells. We show that the promoters of the introduced chimera genes are regulated by their respective trans‐acting factors and may be modulated via membrane receptors and second messengers. This procedure has no noticeable toxic effects, nor does it seem to interfere with complex physiological behavior like neuronal differentiation. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1812 / 1815

页数：4

共 16 条

[1] PHORBOL ESTER INDUCIBLE GENES CONTAIN A COMMON CIS ELEMENT RECOGNIZED BY A TPA-MODULATED TRANS-ACTING FACTOR [J].