RADIOIMMUNOASSAY OF AN ANTIBODY TO PHI-X174 DNA-RNA HYBRID

被引：13

作者：

NAKAZATO, H

机构：

[1] Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90707-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast and simple radioimmunoassay (RIA) technique was developed for an antibody to DNA·RNA hybrid using protein A-bearing Staphylococcus aureus cells as immuno-adsorbent and a glass microfiber filter for the deparation of free antigen and antibody-antigen complex. A simple method for preparing 3H-labeled DNA·RNA hybrid using single-stranded circular DNA of viruses and Escherichia coli RNA polymerase (nucleosidetri-phosphate: RNA nucleotidyltransferase, EC 2.7.7.6) is also described. In comparison to a hybrid made of natural sequences, a synthetic homopolymer hybrid poly (A)·poly(dT) was found to be a poor competitor for the antibody by this RIA technique. © 1979.

引用

页码：74 / 80

页数：7

共 21 条

[1] IMMUNOLOGY OF DNA .2. EFFECT OF SIZE AND STRUCTURE OF ANTIGEN ON FARR ASSAY [J].