CHARACTERIZATION OF A RETINOIC ACID RESPONSIVE ELEMENT ISOLATED BY WHOLE GENOME PCR

We have used whole genome PCR in an attempt to isolate novel retinoic acid (RA) responsive genes. We cloned several small genomic fragments from total human DNA containing putative retinoic acid responsive elements (RAREs) selected by direct binding to the retinoic acid receptor-alpha (RAR-alpha). We report here that an oligonucleotide containing a sequence from one of the cloned human DNA fragments, and referred to as alpha-1, functions as an authentic RARE. It is shown that both RAR-alpha and RAR-beta produced in Cos cells as well as in vitro translated RAR-alpha bind directly and sequence-specifically to the alpha-1RARE. By mutational analysis it is demonstrated that the alpha-1RARE consists of an imperfect direct repeat of the estrogen- and thyroid hormone-related AGGTCA half-site motif separated by a 5 bp spacer. The orientation and spacing of the half-site repeats are shown to play a critical role in RAR recognition. When cloned upstream of a TK-Luc reporter, the alpha-1RARE is shown to confer responsiveness to RA in an orientation-independent fashion in F9 and CV-1 cells. The magnitude of the RA response mediated by the alpha-1RARE differed in these cell lines.