TRANSCRIPTION AND INVIVO EXPRESSION OF A MICROCYSTIS-AERUGINOSA PLASMID

A restriction map of the plasmid pMA1 of Microcystis aeruginosa is presented. The total RNA of the plasmid-containing strain HUB 5-2-4 was isolated and hybridized with the resident plasmid, pMA1, of the strain HUB 5-2-4. A transcript of 1800 b was detected. The minicell-producing strain X 984 of Escherichia coli was transformed with pMA1 cloned into pUC18. Two polypeptides sized 50 kD and 43 kD respectively were synthesized in a specific manner in transformed cells; this indicates that the cyanobacterial transcription and translation signals are recognized by E. coli. © 1990 Springer-Verlag New York Inc.