DOUBLE WAVELENGTH MEASUREMENT OF 3,3',5,5'-TETRAMETHYLBENZIDINE (TMB) PROVIDES A 3-FOLD ENHANCEMENT OF THE ELISA MEASURING RANGE

被引：28

作者：

MADERSBACHER, S ^{[1
]}

BERGER, P ^{[1
]}

机构：

[1] AUSTRIAN ACAD SCI,IMMUNOENDOCRINOL RES UNIT,FRITZ PREGL STR 3IV,A-6020 INNSBRUCK,AUSTRIA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 138卷 / 01期

关键词：

ELISA; ASSAY RANGE; HUMAN CHORIONIC GONADOTROPIN; FREE ALPHA-SUBUNIT; 3,3',5,5'-TETRAMETHYLBENZIDINE; HUMAN FOLLICLE STIMULATING HORMONE;

D O I：

10.1016/0022-1759(91)90071-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, simple method is described which permits a three-fold enhancement of the working range of ELISA procedures using TMB as a substrate. This consists of measuring absorbance values at a wavelength away from the absorption maximum of TMB and using a predetermined multiplication factor.

引用

页码：121 / 124

页数：4

共 5 条

[1] ANTIGENIC FEATURES OF HUMAN FOLLICLE-STIMULATING HORMONE DELINEATED BY MONOCLONAL-ANTIBODIES AND CONSTRUCTION OF AN IMMUNORADIOMOMETRIC ASSAY
BERGER, P
PANMOUNG, W
KHASCHABI, D
MAYREGGER, B
WICK, G
[J]. ENDOCRINOLOGY, 1988, 123 (05) : 2351 - 2359
[2] MONOCLONAL-ANTIBODIES AGAINST THE FREE SUBUNITS OF HUMAN CHORIONIC-GONADOTROPIN
BERGER, P
KLIEBER, R
PANMOUNG, W
MADERSBACHER, S
WOLF, H
WICK, G
[J]. JOURNAL OF ENDOCRINOLOGY, 1990, 125 (02) : 301 - 309
[3] Bos E S, 1981, J Immunoassay, V2, P187, DOI 10.1080/15321818108056977
[4] Nakane M. B., 1978, IMMUNOFLUORESCENCE R, P215
[5] TIJSSEN P, 1985, LABORATORY TECHNIQUE, V15

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