PREPARATION AND CHARACTERIZATION OF MIXED FUNCTIONAL PHASE SILICA MATERIALS USING PHENYLCHLOROSILANE, BUTYLCHLOROSILANE OR OCTYLCHLOROSILANE AS A SILYLATING AGENT

Mixed functional phase (MFP) silica materials having phenyl diol. butyl diol and octyl-diol phases were prepared for direct injection analysis of drugs in serum. The MFP materials were synthesized from a porous silica gel of 9 nm pore size in two steps; introduction of a hydrophobic (phenyl. butyl or octyl) phase using the corresponding trichlorosilane as a silylating agent and introduction of a glycerylpropyl (i.e., diol) phase using 3-glycyldoxypropyltrimethoxysilane in an aqueous medium (pH 3.5). By changing the density of the hydrophobic and hydrophilic ligands, proteins were completely recovered from the prepared MFP materials in the first injection. The MFP materials can be used for direct injection analysis of hydrophobic and hydrophilic drugs in serum.