TRANSGENIC PRODUCTION OF A VARIANT OF HUMAN TISSUE-TYPE PLASMINOGEN-ACTIVATOR IN GOAT MILK - GENERATION OF TRANSGENIC GOATS AND ANALYSIS OF EXPRESSION

被引：204

作者：

EBERT, KM

SELGRATH, JP

DITULLIO, P

DENMAN, J

SMITH, TE

MEMON, MA

SCHINDLER, JE

MONASTERSKY, GM

VITALE, JA

GORDON, K

机构：

[1] GENZYME CORP,FRAMINGHAM,MA 01701

[2] TUFTS UNIV,SCH MED,N GRAFTON,MA 01536

[3] TUFTS UNIV,SCH DENT MED,N GRAFTON,MA 01701

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 09期

关键词：

D O I：

10.1038/nbt0991-835

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA - longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic goats were identified from 29 animals born. The first animal, a female, was mated and allowed to carry the pregnancy to term. Milk was obtained upon parturition and was shown to contain enzymatically active LAtPA at a concentration of 3-mu-g/ml.

引用

页码：835 / 838

页数：4

共 19 条

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