EXPRESSION OF CD4-LIKE STRUCTURE ON MURINE EGG VITELLINE MEMBRANE AND ITS SIGNAL TRANSDUCTIVE ROLES THROUGH P56LCK IN FERTILIZATION

被引：20

作者：

MORI, T

WU, GM

MORI, E

机构：

[1] Department of Immunology, Institute of Medical Science, University of Tokyo, Tokyo

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1991年 / 26卷 / 03期

关键词：

MHC CLASS-II MOLECULE; SPERM; CD4-LIKE MOLECULE; P56LCK; EGG;

D O I：

10.1111/j.1600-0897.1991.tb00704.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Expression of CD4-like molecule on vitelline membrane of murine eggs was demonstrated by indirect immunofluorescence (IIF) test and immunoprecipitation corresponding to the expression of major histocompatibility complex (MHC) class II molecule on murine sperm detected by immunoblotting. This molecule showed slightly larger size than that of the authentic CD4 molecule from T-cells on SDS-PAGE. This molecule was suggested to bind to MHC class II structure on sperm during fertilization because anti-CD4 monoclonal antibody (mAb) blocked in vitro fertilization (IVF). In addition, src-related tyrosine protein kinase (p56lck) was demonstrated in the inner vitelline membrane of eggs by means of IIF with anti-p56lck mAb and immune-complex kinase assay. This molecule was suggested to be associated with CD4-like molecule.

引用

页码：97 / 103

页数：7

共 37 条

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ANDERSON DJ, 1982, J IMMUNOL, V129, P452

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