BIOSYNTHESIS OF WHITE LUPIN ISOFLAVONOIDS FROM [U-C-14]L-PHENYLALANINE AND THEIR RELEASE INTO THE CULTURE-MEDIUM

被引:24
作者
GAGNON, H
SEGUIN, J
BLEICHERT, E
TAHARA, S
IBRAHIM, RK
机构
[1] CONCORDIA UNIV,DEPT BIOL,PLANT BIOCHEM LAB,MONTREAL H3G 1M8,QUEBEC,CANADA
[2] HOKKAIDO UNIV,FAC AGR,DEPT AGR CHEM,SAPPORO,HOKKAIDO 060,JAPAN
关键词
D O I
10.1104/pp.100.1.76
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Pulse-labeling experiments of white lupin (Lupinus albus L.) cell cultures with [U-C-14]L-phenylalanine for 72 h resulted in the incorporation of the radioactivity into the isoflavone aglucones, glucosides, and prenylated derivatives. Both the aglucones genistein and 2'-hydroxygenistein and their 7-O-glucosides accounted for 85% of the total isoflavonoids identified in the cultured cells and contained 35% of the radioactivity, whereas the prenylated derivatives comprised 15 and 65%, respectively. Almost 20% of the labeled isoflavones of the cellular pool was recovered from the culture medium, 90% of which were monoprenylated and diprenylated derivatives containing 80% of the radioactivity. These results clearly demonstrate the release into the culture medium of a substantial amount of the endogenously synthesized isoflavonoids, especially the prenylated derivatives.
引用
收藏
页码:76 / 79
页数:4
相关论文
共 22 条
  • [1] BARZ W, 1985, ANN P PHYTOCHEM SOC, V25, P307
  • [2] BERLIN J, 1991, Z NATURFORSCH C, V46, P725
  • [3] DIXON RA, 1983, ADV ENZYMOL RAMB, V55, P1
  • [4] NATURAL-PRODUCTS FROM PLANT-TISSUE CULTURE
    ELLIS, BE
    [J]. NATURAL PRODUCT REPORTS, 1988, 5 (06) : 581 - 612
  • [5] GAGNON H, 1992, IN PRESS J CHROMATOG
  • [6] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS
    GAMBORG, OL
    MILLER, RA
    OJIMA, K
    [J]. EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) : 151 - +
  • [7] Guern J, 1987, CELL CULTURE SOMATIC, P43
  • [8] ISOPENTENYL ISOFLAVONE LUTEONE AS A PRE-INFECTIONAL ANTIFUNGAL AGENT IN GENUS LUPINUS
    HARBORNE, JB
    INGHAM, JL
    KING, L
    PAYNE, M
    [J]. PHYTOCHEMISTRY, 1976, 15 (10) : 1485 - 1487
  • [9] IBRAHIM RK, 1987, CELL CULTURE PHYTOCH, V4, P77
  • [10] INGHAM JL, 1983, Z NATURFORSCH C, V38, P194