RAINBOW-TROUT OVARIAN CHOLESTEROL SIDE-CHAIN CLEAVAGE CYTOCHROME-P450 (P450SCC) - CDNA CLONING AND MESSENGER-RNA EXPRESSION DURING OOGENESIS

被引：49

作者：

TAKAHASHI, M

TANAKA, M

SAKAI, N

ADACHI, S

MILLER, WL

NAGAHAMA, Y

机构：

[1] NATL INST BASIC BIOL,REPROD BIOL LAB,OKAZAKI,AICHI 444,JAPAN

[2] GRAD UNIV ADV STUDIES,DEPT MOLEC BIOMECH,OKAZAKI 444,JAPAN

[3] UNIV CALIF SAN FRANCISCO,DEPT PEDIAT,SAN FRANCISCO,CA 94143

[4] UNIV CALIF SAN FRANCISCO,METAB RES UNIT,SAN FRANCISCO,CA 94143

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

RAINBOW TROUT; CYTOCHROME P450SCC; EXPRESSION IN COS-1 CELLS; STEROIDOGENESIS;

D O I：

10.1016/0014-5793(93)80034-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with that of human, and 46% homology with that of rat, bovine and pig. P450scc activity was confirmed by transfected COS-1 monkey kidney tumour cells with an expression vector for trout P450scc cDNA and subsequent detection of conversion from 25-hydroxycholesterol to pregnenolone by radioimmunoassay. The cDNA only hybridized to a single 1.8 kb RNA transcript. The transcript was not found in early vitellogenic follicles, barely detected in postvitellogenic follicles, and abundant in postovulatory follicles.

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页码：45 / 48

页数：4

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