MICROBORE HPLC DETERMINATION OF POLYETHER ANTIBIOTICS USING POSTCOLUMN DERIVATIZATION WITH BENZALDEHYDE REAGENTS

被引：9

作者：

FEJGLOVA, Z ^{[1
]}

DOLEZAL, J ^{[1
]}

HRDLICKA, A ^{[1
]}

FRGALOVA, K ^{[1
]}

机构：

[1] MASARYK UNIV,DEPT ANALYT CHEM,CS-61137 BRNO,CZECH REPUBLIC

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 02期

关键词：

D O I：

10.1080/10826079408013357

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monensin, narasin, and salinomycin have been separated by HPLC on microbore column packed with octadecyl-bonded silica gel and detected via postcolumn color reaction with derivative of benzaldehyde in packed-bed reactor. Various benzaldehyde reagents have been tested in batch and 4-dimethylaminobenzaldehyde (DMABA) and vanillin selected as most promising ones. Requirements and characteristics of postcolumn reaction with the two reagents have been investigated and optimized and DMABA has been shown as superior over currently used vanillin reagent. The applicability of postcolumn derivatization with DMABA has been demonstrated by monensin assay in premixes and feeds.

引用

页码：359 / 372

页数：14

共 9 条

[1] BLANCHFLOWER WJ, 1985, ANALYST, V110, P1283, DOI 10.1039/an9851001283
[2] THE KOMAROWSKI REACTION
DUKE, FR
[J]. ANALYTICAL CHEMISTRY, 1947, 19 (09) : 661 - 662
[3] GOLAB T, 1973, J ASSOC OFF ANA CHEM, V56, P171
[4] GORAS JT, 1984, J ASSOC OFF ANA CHEM, V67, P701
[5] Komarowski A, 1903, CHEM ZTG, V27, P1806
[6] KOMAROWSKY A, 1903, CHEM ZTG, V27, P807
[7] LAPOINTE MR, 1988, J ASSOC OFF ANA CHEM, V71, P480
[8] RODEWALD JM, 1992, J AOAC INT, V75, P272
[9] RODEWALD JM, 1984, AMAACAJ199AA755 LILL

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