A TRIGONAL FORM OF THE IDARUBICIN-D(CGATCG) COMPLEX - CRYSTAL AND MOLECULAR-STRUCTURE AT 2.0 ANGSTROM RESOLUTION

被引:28
作者
DAUTANT, A
DESTAINTOT, BL
GALLOIS, B
BROWN, T
HUNTER, WN
机构
[1] UNIV MANCHESTER,DEPT CHEM,MANCHESTER M13 9PL,LANCS,ENGLAND
[2] UNIV BORDEAUX 1,CRISTALLOG LAB,CNRS,ERS 133,F-33405 TALENCE,FRANCE
[3] UNIV EDINBURGH,DEPT CHEM,EDINBURGH,MIDLOTHIAN,SCOTLAND
基金
英国惠康基金;
关键词
D O I
10.1093/nar/23.10.1710
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The X-ray crystal structure of the complex between the anthracycline idarubicin and d(CGATCG) has been solved by molecular replacement and refined to a resolution of 2.0 Angstrom. The final R-factor is 0.19 for 3768 reflections with F-o greater than or equal to 2 sigma(F-o). The complex crystallizes in the trigonal space group P3(1) with unit cell parameters a = b = 52.996(4), c = 33.065(2) alpha, alpha = beta = 90 degrees, gamma = 120 degrees. The asymmetric unit consists of two-duplexes, each one being complexed with two idarubicin drugs intercalated at the CpG steps, one spermine and 160 water molecules. The molecular packing underlines major groove-major groove interactions between neighbouring helices, and an unusually low value of the occupied fraction of the unit cell due to a targe solvent channel of similar to 30 Angstrom diameter. This is the first trigonal crystal form of a DNA-anthracycline complex. The structure is compared with the previously reported structure of the same complex crystallizing in a tetragonal form. The geometry of both the double helices and the intercalation site are conserved as are the intramolecular interactions despite the different crystal forms.
引用
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页码:1710 / 1716
页数:7
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