CHARACTERIZATION OF CYTOCHROME C FROM NITROBACTER AGILIS

被引：14

作者：

KETCHUM, PA

SANDERS, HK

GRYDER, JW

NASON, A

机构：

[1] McCollum-Pratt Institute, the Department of Chemistry, The Johns Hopkins University, Baltimore

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 189卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0005-2728(69)90167-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytochrome c from Nitrobacter agilis was isolated and purified approx. 60-fold. Absorption spectra of both the oxidized and the reduced Nitrobacter cytochrome c and the oxidized minus reduced difference spectrum of this cytochrome were essentially identical to the corresponding spectra of horse-heart cytochrome c. The redox potential of this cytochrome was determined by spectrophotometric titration with ferrocyanide/ferricyanide and found to be +0.282 V over the pH range 6.0 to 8.7, while a potential of +0.265 V was determined in the same manner for horse-heart cytochrome c. The titration also indicated that the Nitrobacter ferrocytochrome is oxidized by a single electron transfer. © 1969.

引用

页码：360 / &

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