Two new C28 steroidal glycosides called daturataturin A (1a) and daturataturin B (2a) were isolated from the methanolic extract of the fresh aerial parts of Datura tatura L. (Solanaceae), and their chemical structures were characterized as (22R)-7-alpha,27-dihydroxy-1-oxowitha-2,5,24,trienolide 27-O-beta-D-glucopyranoside and (22R)-1-alpha,3-beta-7-alpha,-27-tetrahydroxywitha-5,24-dienolide 3-O-beta-D-glucopyranoside by spectral analysis.