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SEQUENCE CONSERVATION IN THE ALPHA-SUBUNIT AND BETA-SUBUNIT OF PYRUVATE-DEHYDROGENASE AND ITS SIMILARITY TO BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE

被引：54
作者：
WEXLER, ID
HEMALATHA, SG
PATEL, MS
机构：
[1] CASE WESTERN RESERVE UNIV, SCH MED, DEPT BIOCHEM, CLEVELAND, OH 44106 USA
[2] CASE WESTERN RESERVE UNIV, SCH MED, DEPT PEDIAT, CLEVELAND, OH 44106 USA
来源：
FEBS LETTERS | 1991年 / 282卷 / 01期
关键词：
PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE COMPLEX; BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX; ALPHA-KETO ACID DEHYDROGENASE COMPLEX; AMINO ACID SEQUENCE COMPARISON;
D O I：
10.1016/0014-5793(91)80479-M
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Amino acid sequence comparison of 8 alpha and 6 beta subunits of the alpha-keto acid dehydrogenase (E1) component of the pyruvate dehydrogenase complex and branched-chain alpha-keto acid dehydrogenase complex from multiple species was performed by computer analysis. In addition to 2 previously recognized regions of homology in the alpha subunit, a 3rd region of extensive homology was identified in E1-alpha, and may be one of the sites involved in subunit interaction, E1-beta contains 4 regions of extensive homology. Region 1 contains 10 amino acids that are homologous to a 10-amino acid stretch in Escherichia coli E1. Regions 2 and 3 have sequence homologies with other dehydrogenases suggesting that these regions may be involved in catalysis.
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页码：209 / 213
页数：5
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