MORTALITY OF FECAL BACTERIA IN SEAWATER

被引：30

作者：

GARCIALARA, J ^{[1
]}

MENON, P ^{[1
]}

SERVAIS, P ^{[1
]}

BILLEN, G ^{[1
]}

机构：

[1] UNIV LIBRE BRUXELLES,MICROBIOL MILIEUX AQUATIQUES GRP,CAMPUS PLAINE,BLVD TRIOMPHE,B-1050 BRUSSELS,BELGIUM

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 03期

关键词：

D O I：

10.1128/AEM.57.3.885-888.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We propose a method for determining the mortality rate for allochthonous bacteria released in aquatic environments without interference due to the loss of culturability in specific culture media. This method consists of following the disappearance of radioactivity from the trichloroacetic acid-insoluble fraction in water samples to which [H-3]thymidine-prelabeled allochthonous bacteria have been added. In coastal seawater, we found that the actual rate of disappearance of fecal bacteria was 1 order of magnitude lower than the rate of loss of culturability on specific media. Minor adaptation of the procedure may facilitate assessment of the effect of protozoan grazing and bacteriophage lysis on the overall bacterial mortality rate.

引用

页码：885 / 888

页数：4

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