THE METHYL-5-ALPHA-DIHYDROTESTOSTERONES MESTEROLONE AND DROSTANOLONE - GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC CHARACTERIZATION OF THE URINARY METABOLITES

被引：27

作者：

DEBOER, D

DEJONG, EG

MAES, RAA

VANROSSUM, JM

机构：

[1] Netherlands Institute for Drugs and Doping Research, 3584 CA Utrecht

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1992年 / 42卷 / 3-4期

关键词：

D O I：

10.1016/0960-0760(92)90146-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Before including the detection of the methyl-5-alpha-dihydrotestosterones mesterolone (1-alpha-methyl-17-beta-hydroxy-5-alpha-androstan-3-one) and drostanolone (2-alpha-methyl-17-beta-hydroxy-5-alpha-androstan-3-one) in doping control procedures, their urinary metabolites were characterized by gas chromatography/mass spectrometry. Several metabolites were found after enzymatic hydrolysis and conversion of the respective metabolites to their trimethylsilyl-enol-trimethylsilyl ether derivatives. The major metabolites of mesterolone and drostanolone were identified as 1-alpha-methyl-androsterone and 2-alpha-methyl-androsterone, respectively. The parent compounds and the intermediate 3-alpha,17-beta-dihydroxysteroid metabolites were detected as well. The reduction into the corresponding 3-beta-hydroxysteroids was a minor metabolic pathway. All metabolites were found to be conjugated to glucuronic acid.

引用

页码：411 / 419

页数：9

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