CAMP INDUCES UP-REGULATION OF ET(A) RECEPTOR MESSENGER-RNA AND INCREASES RESPONSIVENESS TO ENDOTHELIN-1 OF RAT AORTIC SMOOTH-MUSCLE CELLS IN PRIMARY CULTURE

被引：47

作者：

NISHIMURA, J ^{[1
]}

CHEN, X ^{[1
]}

JAHAN, H ^{[1
]}

SHIKASHO, T ^{[1
]}

KOBAYASHI, S ^{[1
]}

KANAIDE, H ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,ANGIOCARDIOL RES INST,DIV MOLEC CARDIOL,FUKUOKA 812,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91115-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of cAMP on the expression of ETA (ET-1 selective type) receptor mRNA and on the response to endothelin-1 (ET-1) were investigated in rat aortic smooth muscle cells in primary culture, using reverse transcription polymerase chain reaction (RT-PCR) and fura-2 microfluorometry of cytosolic Ca2+ concentrations ([Ca2+]i). Incubation of the smooth muscle cells with 10 μM forskolin and 10 μM 3-isobutyl-1-methyl-xanthine (IBMX) induced an increase in ETA receptor mRNA by 420 % of control after 24 hours. ET-1 induced a biphasic increase in [Ca2+]i, first transient and second sustained phases. Incubation of the smooth muscle cells with forskolin and IBMX for 24 hours increased the [Ca2+]i response by 849 % in the first phase and 286 % in the second phase, compared with time-matched controls. From these results, we conclude that cAMP induces an up-regulation of the ETA receptor mRNA and increases responsiveness to ET-1 of rat aortic smooth muscle cells in primary culture. © 1992.

引用

页码：719 / 726

页数：8

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