METHODS FOR ANIMAL-CELL IMMOBILIZATION USING ELECTROSTATIC DROPLET GENERATION

被引：18

作者：

BUGARSKI, B ^{[1
]}

SMITH, J ^{[1
]}

WU, J ^{[1
]}

GOOSEN, MFA ^{[1
]}

机构：

[1] QUEENS UNIV, DEPT CHEM ENGN, KINGSTON K7L 3N6, ONTARIO, CANADA

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 09期

关键词：

D O I：

10.1007/BF00151869

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique for the production of small polymer microbeads (less than 150 mum diam.) utilizing electrostatic spraying is presented. Video and image analysis were performed to reveal details of the mechanism of droplet formation. Microbeads coated with poly-L-lysine were prepared and found to be effective as microcarriers for culturing surface immobilized insect cells to a high cell density (1.55 x 10(7) cells/ml microbeads).

引用

页码：677 / 682

页数：6

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