5-HT(1A) RECEPTOR-MEDIATED INHIBITION OF N-TYPE CALCIUM CURRENT IN ACUTELY ISOLATED VENTROMEDIAL HYPOTHALAMIC NEURONAL CELLS

被引:23
作者
KOIKE, H [1 ]
SAITO, H [1 ]
MATSUKI, N [1 ]
机构
[1] UNIV TOKYO, FAC PHARMACEUT SCI, DEPT CHEM PHARMACOL, BUNKYO KU, TOKYO 113, JAPAN
关键词
SEROTONIN (5-HT); 8-OH-DPAT; VOLTAGE-GATED CALCIUM CHANNEL (VGCC); VENTROMEDIAL HYPOTHALAMUS; ACUTELY ISOLATED NEURONAL CELL;
D O I
10.1016/0168-0102(94)90139-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The effects of serotonin (5-hydroxytryptamine: 5-HT) and 5-HT1A agonist on voltage-gated calcium channels (VGCCs) in acutely isolated ventromedial hypothalamic (VMH) neuronal cells were studied using whole-cell configuration of the patch-clamp technique. 5-HT at 10 muM inhibited inward calcium current reversibly in 80% of cells. This inhibition was specific to N-type current. Because pindolol blocked the effect of 5-HT and 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) mimicked the effect of 5-HT, the inhibitory effect of 5-HT appeared to be mediated via the 5-HT1A receptor. In the fura-2 fluorometry method, 8-OH-DPAT DPAT attenuated the [Ca2+]i increase induced by the depolarization stimulus of 50 mM K+. These results indicate that 5-HT suppresses Ca2+ entry through N-type channels in the VMH neurons via the 5-HT1A receptor and that the stimulating effect of 8-OH-DPAT on feeding behavior may be mediated by the blocking of Ca2+ entry through N-type channels.
引用
收藏
页码:161 / 166
页数:6
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